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KMID : 0380020070220060443
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 6 p.443 ~ p.446
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Detection of C-Reactive Protein Using Direct-binding Quartz Crystal Microbalance Immunosensor
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Kim Nam-Soo
Cho Young-Jin
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Abstract
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A prognostic indicator of coronary heart disease, C-reactive protein, was tried to be determined by a batch-type quartz crystal microbalance immunosensor. The sensor was operated by direct-binding mode and the optimum concentration for the corresponding antibody for immobilization was . The reaction buffer for the system was 0.1 M sodium phosphate (pH 7.0) and system operation was performed in the order of baseline stabilization, analyte addition and measurement, and regeneration of the sensor chip with 10 mM NaOH. When plotted in double-logarithmic scale, the sensor showed a linear detection range of 0.27-106.00 nM for rat C-reactive protein with the limit of detection of 0.53 nM. It also showed a good reusability.
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KEYWORD
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C-Reactive protein, quartz crystal microbalance, immunosensor, linearity, reusability
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